|
KMID : 0613820190290090972
|
|
Journal of Life Science
2019 Volume.29 No. 9 p.972 ~ p.976
|
|
|
Inhibitory Effects of Polyopes affinis Ethanol Extract on Melanogenesis in B16F10 Melanoma Cells
|
|
Kim Hyang-Suk
Choi Yung-Hyun
Hwang Hye-Jin
|
|
|
Abstract
|
|
|
|
Polyopes affinis is a kind of red algae found in the South coast and near Jeju Island of Korea. The purpose of this study was to investigate the effects of Polyopes affinis ethanol extract (PAEE) on melanogenesis in α-MSH stimulated B16F10 melanoma cells. Melanoma cells were cultured for 72 hr treated with PAEE. Total melanin content and the activity of tyrosinase, a key enzyme in melanogenesis, were measured. When the melanin content in B16F10 melanoma cells was tested, PAEE was decreased in a dose-dependent manner: treatment with 100, 300, and 500 μg/ml caused 25%, 30%, and 35% reduction, respectively. Treatment of 100, 300, and 500 μg/ml of PAEE caused 6%, 12%, and 21% reduction of tyrosinase activities in B16F10 melanoma cells. Also, PAEE suppressed the expression of tyrosinase, tyrosinase-related protein-1, tyrosinase-related protein-2, and melanocyte-inducing transcription factor in B16F10 melanoma cells. A concentration of 500 μg/ml of PAEE showed a greater decrease in tyrosinase activity, melanin content, and melanogenic enzyme protein expression. These results indicate that PAEE inhibits melanin synthesis and tyrosinase activity, and Polyopes affinis ethanol extract could be used as a functional whitening agent.
|
|
|
KEYWORD
|
|
|
Melanogenesis, Polyopes affinis, tyrosinase, whitening agent
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|